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Updated: Aug 15, 2026

Automated Hydrophobic Interaction Chromatography Column Selection for Use in Protein Purification
Published on: September 21, 2011
[Preparation and evaluation of wide-bore stainless-steel packed capillary liquid chromatographic columns]
1Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, China.
Abstract:
A zero-dead volume column end and a conical shaped slurry reservoir for wide-bore stainless-steel packed capillary liquid chromatographic columns were designed and evaluated. A detailed procedure for the preparation of reversed-phase stainless-steel packed capillary columns with 0.5 mm-1.0 mm i.d. is described. The influences of the column length and the packing material on the column performance have been studied. Columns were evaluated by the reduced plate height vs reduced velocity curve and the peak asymmetric factor. Experimental results showed that the column efficiency and the reproducibility were better than 75% of theoretical value and 6% RSD, respectively. Separations of antiepileptic drugs and chlorinated benzenes are demonstrated.
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