Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Aptamers as tools for target prioritization and lead identification.

Petra Burgstaller1, Anne Girod, Michael Blind

  • 1NasaCell, Bahnhofstrasse 9-15, 82327 Tutzing, Germany.

Drug Discovery Today
|January 28, 2003
PubMed
Summary

Aptamers, or synthetic nucleic acid ligands, are revolutionizing drug discovery by enabling target validation and high-throughput screening (HTS). These molecules efficiently identify drug targets and facilitate the discovery of new lead compounds.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Aptamer Selection Technology and Recent Advances.

Molecular therapy. Nucleic acids·2017
Same author

Positive Modulation of the Glycine Receptor by Means of Glycine Receptor-Binding Aptamers.

Journal of biomolecular screening·2015
Same author

Selective aptamer-based control of intraneuronal signaling.

Angewandte Chemie (International ed. in English)·2015
Same author

An aptamer to the MAP kinase insert region.

ACS chemical biology·2014
Same author

In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin.

Biochemical and biophysical research communications·2010
Same author

From selection to caged aptamers: identification of light-dependent ssDNA aptamers targeting cytohesin.

Bioorganic & medicinal chemistry letters·2009

Area of Science:

  • Biotechnology and Pharmaceutical Sciences
  • Molecular Biology and Drug Discovery

Background:

  • The growing number of potential drug targets necessitates advanced technologies for target identification and lead discovery.
  • Synthetic nucleic acid ligands, known as aptamers, offer a novel approach to drug discovery.

Purpose of the Study:

  • To highlight the utility of aptamers in validating potential drug targets.
  • To demonstrate how aptamers can be integrated into high-throughput screening (HTS) for lead compound identification.
  • To showcase aptamers as tools for modulating protein function in cellular environments and disease models.

Main Methods:

  • Development and application of aptamer-based technologies for target validation.
  • Utilizing aptamers as specific antagonists to modulate endogenous protein function.

Related Experiment Videos

  • Employing aptamers as interaction partners in HTS assays.

    • Main Results:

    • Aptamers effectively link target validation with HTS, streamlining the drug discovery pipeline.
    • Aptamers have been proven valuable in modulating protein function within their natural cellular context.
    • Aptamer-specific technologies enable target validation in various disease models.

    Conclusions:

    • Aptamers represent a significant advancement in drug discovery, offering a versatile platform for target identification and validation.
    • The use of aptamers in HTS assays accelerates the identification of small-molecule lead compounds.
    • Aptamer technology holds great promise for developing novel therapeutics by enabling precise modulation of protein targets.