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Related Experiment Video

Updated: Mar 8, 2026

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Aptamer Selection Technology and Recent Advances.

Michael Blind1, Michael Blank1

  • 1AptaIT GmbH, Munich, Germany.

Molecular Therapy. Nucleic Acids
|January 24, 2017
PubMed
Summary
This summary is machine-generated.

Aptamers are increasingly used in diagnostics due to their stability. This review details advancements in aptamer selection technology (SELEX), focusing on next-generation sequencing and bioinformatics for efficient aptamer development.

Keywords:
NGSSELEXaptamerbioinformaticsmicrofluidicsnext generation sequencing

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Diagnostics

Background:

  • Aptamers, short nucleic acid sequences, have transitioned from research to commercial applications.
  • Diagnostic applications are more widespread than clinical uses due to aptamers' inherent stability and pharmacokinetic properties.
  • The in vitro selection technology (SELEX) has seen significant technical advancements to improve aptamer development efficiency.

Purpose of the Study:

  • To review recent technical progress in SELEX experiments.
  • To highlight the role of high-throughput next-generation sequencing and bioinformatics in SELEX.
  • To discuss achievements in optimizing aptamer libraries, separation, amplification, and sequence identification.

Main Methods:

  • Focus on advancements in the technical steps of SELEX.
  • Integration of high-throughput next-generation sequencing for aptamer library analysis.
  • Application of bioinformatics tools for aptamer sequence identification and validation.

Main Results:

  • Improvements in aptamer library design and construction.
  • Enhanced separation techniques for isolating target-bound aptamers.
  • Streamlined amplification and sequencing processes for enriched aptamer libraries.
  • Successful identification of aptamer sequences from complex libraries.

Conclusions:

  • Technical progress in SELEX, particularly with next-generation sequencing and bioinformatics, enhances aptamer development.
  • Optimized SELEX steps lead to more efficient identification of high-affinity aptamers for commercial targets.
  • These advancements facilitate the broader application of aptamers in diagnostics and other fields.