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Updated: Sep 27, 2026
Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model
Published on: April 2, 2017
Neutrophil elastase inhibitor reduces hepatic metastases induced by ischaemia-reperfusion in rats
Koji Doi1, Tetsuya Horiuchi, Masaru Uchinami
1Second Department of Surgery, Fukui Medical University, Yoshida-gun, Fukui, Japan.
Objective:
To investigate the possibility in rats that ONO-5046 Na, a new recombinant inhibitor of neutrophil elastase, can reduce hepatic metastases induced by ischaemia-reperfusion.
Design:
Laboratory experimental study.
Setting:
Research laboratory, Japan.
Subjects:
Male Fischer rats.
Interventions:
Rats underwent 60 min of 70% partial hepatic ischaemia, after which rat colon adenocarcinoma cells (RCN-H4) were injected into the spleen. The animals were divided into two test groups and a control group. One group was given ONO-5046 Na intravenously at 10 mg/kg/hour. A second group was given a saline solution for the same period, while the controls were not made ischaemic.
Main Outcome Measures:
Three weeks after inoculation, the number of tumour nodules on the liver surface was counted. The anti-cancer effect of ONO-5046 Na was measured by monotetrazolium assay.
Results:
Hepatic ischaemia-reperfusion increased the number of liver metastases of RCN-H4 in both clamped and unclamped hepatic lobes. ONO-5046 Na significantly inhibited this in unclamped lobes, but had no anti-cancer effect.
Conclusion:
Neutrophil elastase may have an important role in increasing haematogenous liver metastases by ischaemia-reperfusion, particularly in unclamped lobes.
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