[Determination of acemetacin and indometacin in human serum by high performance liquid chromatography]
1Department of Pharmacology, Bethune International Peace Hospital, Shijiazhuang 050082, China.
Abstract:
A method of HPLC for the quantitative determination of acemetacin and indometacin in human serum is described. After being extracted with diethyl ether, acemetacin and indometacin were analyzed by reversed-phase HPLC(Spherisorb-C8) and UV-detector(254 nm), with tolbutamide as internal standard. The mobile phase was a mixture of V(acetate buffer solution, pH 4.6):V(methyl alcohol):V(acetonitrile) = 55:5:40 and at a rate of 1.0 mL/min. Over the mass concentration range of 12.5 micrograms/L-1.6 mg/L, both the calibration curves were linear, r = 0.9996, n = 8. The average recoveries of acemetacin and indometacin were 77.2% and 86.7% respectively. The within-day and between-day RSD of acemetacin and indometacin were less than 5% and 10% respectively.
More Related Videos
10:17High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
07:34Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)
Published on: March 14, 2013
Related Concept Videos
01:15High-Performance Liquid Chromatography: Types of Detectors
01:22Protein-Drug Binding: Determination Methods
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...