[Simultaneous determination of gibberellins A1 and A3 in foxtail millet seedlings by high performance liquid...

Gibberellin A1(GA1) and Gibberellin A3(GA3) in millet seedlings were simultaneously determined by HPLC on a Zorbax-ODS-C18 (4.6 mm x 250 mm, 10 microns) column, using a mobile phase consisting of V (0.02 mol/L phosphate buffer solution, pH 4.0):V(methanol) = 2.2:1, a flow rate of 0.8 mL/min and a UV detection at 210 nm. GA1 and GA3 were separated completely in 10 min. Correlation coefficients for linear equations were 0.9986 and 0.9978, recoveries were 102.54% and 99.59%, respectively. Two normal cultivars and their dwarf-mutants were determined by this method. The results showed that both GA1 and GA3 were discovered in foxtail millet seedling, and endogenous mass ratio of GA1 and GA3 in foxtail millet dwarf-mutants were significantly lower than those in normal cultivars, and the concentrations of endogenous GA1 and GA3 were important factors for plant-height.