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Updated: Aug 2, 2026

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
[The cloning and functional analysis of Sinorhizobium fredii 042BS regulatory modulation genes]
Haiyu Zhang1, Haiyu Zhang, Xiaohong Li
1Department of Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.
Abstract:
The total DNA of Sinorhizobium fredii 042BS was digested by EcoRI for Southern blotting with probes of nodD1 and nodD2 from S. fredii USDA257. The 3 kb positive band hybrided with nodD1 probe and 6 kb positive band with nodD2 probe were found, respectively. Partial gene library were constructed using pUC18 as vector, and the clones with the nodD1 and nodD2 genes were obtained. The sequence of nodD1 and nodD2 of 042B showed that they are highly homologous with nodD1 and nodD2 of S. fredii. The fragment with nodD1 was cloned into the vector pBBRIMCS-5 and introduced into R. leguminosarum bv. viciae LPR5054 to study the function of the nodD1. The results showed that nodD1 of 042B can be induced by genistein and luteolin secreted by the seedlings of soybean and alfalfa respectively.
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