[Study on amount and stability of cry1D and cry1Ab mRNA in Bacillus thuringiensis subps aizawai]

cry1Ab, cry1C and cry1D are three typical cry1 class genes encoding protoxins in Bacillus thuringiensis subsp. aizawai strain HD-133. The expression of cry1D is obviously different from that of cry1Ab in this strain. Amount and stability of cry1D and cry1Ab mRNA in HD-133 were investigated. Northern blotting analysis showed that cry1D mRNA was more stable than cry1Ab mRNA, however, cry1D mRNA formed later 3 h and less 3.7 times than cry1Ab mRNA during the mid-phase of sporulation. Halve lives of cry1D mRNA was 18 min, but halve lives of cry1Ab mNRA was 14 min. Moreover, cry1Ab mRNA could keep stable and large amount during the post-phase of sporulation. This suggested that difference of transcriptional efficiency and initiate time might be main reason for the difference of expression of cry1Ab and cry1D.