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[The cell wall-degrading activities of lysin encoded by chlorella virus PBCV-1]
Jigang Han1, Pingfang Liu, Ming Kang
1College of Life Science, Hebei Universisty, Baoding 071002, China.
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|February 1, 2003
Abstract:
Lysin was isolated from Chlorella NC64A lysate which was infected by Chlorella virus PBCV-1. The enzyme property detection of lysin shows that it contains at least three enzyme activities: chitinase, chitosanase and beta-1, 3-Glucanase. This is consistent with the compose of Chlolrella cell wall. Chitinase and chitosanase, especially chitinase plays a important role in the process of virus entry. One 52 kD chitinase, one 56 kD chitosanase and one 36 kD chitosanase were obtained after purified by FPLC.