Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyamine transport, accumulation, and release in brain.

Takashi Masuko1, Kuniko Kusama-Eguchi, Kaori Sakata

  • 1Graduate School of Pharmaceutical Sciences, Chiba University, Chiba, Japan.

Journal of Neurochemistry
|February 1, 2003
PubMed
Summary

Brain polyamine transport involves distinct systems in glial cells, neurons, and synaptic vesicles. These systems, particularly spermine transport, suggest polyamines may act as brain neuromodulators.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inbred strains of Xenopus tropicalis show morphological and genetic variation.

Scientific reports·2026
Same author

Identification of Inhibitors for eIF5A2-Dependent Translation Elongation by Monitoring the Translational Efficiency of Polyproline Motif in Mitochondrial Fission Regulator 1.

Biological & pharmaceutical bulletin·2026
Same author

Inshore marine coastal zone migration patterns in Atlantic salmon post-smolts emigrating from eight rivers in north-east Scotland.

Journal of fish biology·2025
Same author

Polyamines stimulate the protein synthesis of the translation initiation factor eIF5A2, participating in mRNA decoding, distinct from eIF5A1.

The Journal of biological chemistry·2025
Same author

Anticancer agent doxorubicin toxicity to in vitro X. laevis tadpole organ heart and mature frog heart and liver mitochondria.

The Journal of toxicological sciences·2025
Same author

Measurements of acrolein adducts resulting from polyamine catabolism.

Methods in enzymology·2025

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Biochemistry

Background:

  • Polyamines, such as spermine and spermidine, are crucial for cellular functions.
  • Their role and transport mechanisms within the brain remain incompletely understood.

Purpose of the Study:

  • To investigate the cycling and transport of polyamines in different brain compartments.
  • To elucidate the specific properties of polyamine transporters in glial cells, synaptosomes, and synaptic vesicles.
  • To explore the potential role of spermine as a neuromodulator.

Main Methods:

  • Measurement of polyamine transport in isolated synaptic vesicles, synaptosomes, and glial cells.
  • Analysis of spermine release from rat hippocampal slices upon depolarization.
  • Characterization of transporter affinities and inhibition patterns.

Related Experiment Videos

Main Results:

  • Distinct polyamine transport systems identified: membrane potential-dependent in synaptosomes/glial cells, proton gradient-dependent in synaptic vesicles.
  • Transporter affinities varied, with synaptic vesicles and synaptosomes showing higher affinity for spermine over spermidine.
  • Specific inhibitors differentially affected transport in each compartment, suggesting unique molecular entities.

Conclusions:

  • Glial cells, neurons, and synaptic vesicles possess unique polyamine transporters.
  • Spermine accumulation in synaptic vesicles and its release upon depolarization support its potential role as a brain neuromodulator.