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Updated: Jul 30, 2026
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Published on: July 6, 2012
A novel application of thermo-responsive polymer to affinity precipitation of polysaccharide
Li-Chun Pan1, Chih-Ching Chien
1Biomedical Engineering Center, Industrial Technology Research Institute, Chutung, Hsinchu 310, Taiwan, ROC.
Abstract:
The lectin receptors concanavalin A (ConA) and wheat germ lectin (WGL) were successfully conjugated to a thermo-responsive hydrogel polymer (poly-N-isopropylacrylamide) (PNIPAAm) as an affinity ligand. The coupling efficiencies of the ConA and WGL to PNIPAAm were 5.1% and 44%, respectively. These lectin receptor-polymer conjugates were then tested for their efficiency in purification of various polysaccharides or polysaccharide-containing compounds such as beta-glucan. Results indicated that these conjugates separated various polysaccharides from a complex mixture. The use of a thermo-responsive polymer in low-temperature purification of potentially heat-labile glycoproteins is advantageous. Additionally, other affinity ligands could be coupled to the polymer for separation of the respective bioactive molecules.
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