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Changes of oxidative stress in various tissues by long-term administration of vitamin E in hypercholesterolemic rats
Cahide Gökkuşu1, Tannaz Mostafazadeh
1Department of Biochemistry, Istanbul Faculty of Medicine, University of Istanbul, Capa 34390, Istanbul, Turkey. cahgok@istanbul.edu.tr
Background:
Free radical-mediated oxidative stress has been implicated in the genesis and progression of atherosclerosis.
Methods:
The lipid peroxides and antioxidant status of various tissues were investigated in hypercholesterolemic rats and the effect of vitamin E supplementation on defense systems.
Results:
Cholesterol-feeding caused a significant increase in the lipid peroxide concentrations of plasma, erythrocytes, liver and brain. In addition, a significant decrease in glutathione (GSH) content, glutathione peroxidase (GSH-Px) and glutathione transferase (GSH-ST) activities were found in erythrocytes and liver but superoxide dismutase (SOD) activity remained unchanged in these tissues in comparison to the control group. Vitamin E supplementation to hypercholesterolemic rats induced a significantly decrease in lipid peroxide concentrations and a significant increase in the GSH content, GSH-Px and GSH-ST activities in erythrocytes and liver.
Conclusions:
Long-term administration of vitamin E may play an important role in suppressing oxidative stress, and thus, may be useful for the prevention and/or early treatment of hypercholesterolemia.