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Inorganic plasma with physiological CO2/HCO3- buffer.

P A A P Marques1, M C F Magalhães, R N Correia

  • 1Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal. paulam@dq.ua.pt

Biomaterials
|February 1, 2003
PubMed
Summary
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Researchers replaced Tris/HCl buffer with a carbon dioxide/bicarbonate buffer in simulated inorganic plasma. This new method better mimics biomineralization processes for calcium phosphate ceramics.

Area of Science:

  • Biomaterials Science
  • Biomineralization Research
  • Materials Chemistry

Background:

  • Simulated Body Fluid (SBF) solutions are crucial for studying biomaterial interactions.
  • Traditional SBF often uses Tris/HCl buffer, which differs from physiological conditions.
  • Physiological buffering involves carbon dioxide/bicarbonate (CO(2)/HCO(3)(-)) systems.

Purpose of the Study:

  • To substitute Tris/HCl buffer with a CO(2)/HCO(3)(-) buffer in inorganic plasma.
  • To establish a buffered solution mimicking blood plasma's pH and bicarbonate levels.
  • To investigate the effect of this new buffer system on calcium phosphate ceramic mineralization.

Main Methods:

  • Developed a CO(2)/N(2) gas mixture bubbling system for Kokubo's SBF.
  • Prepared SBF without Tris/HCl, maintaining pH 7.3-7.4 and HCO(3)(-) concentration of 24-27 mmol dm(-3).

Related Experiment Videos

  • Performed mineralization studies on calcium phosphate ceramics in the modified SBF.

    • Main Results:

    • The CO(2)/HCO(3)(-) buffer successfully maintained physiological pH and bicarbonate concentrations.
    • Mineralization on ceramics formed a layer of carbonated octacalcium phosphate.
    • This layer evolved into carbonated hydroxyapatite, indicating successful biomimetic mineralization.

    Conclusions:

    • The developed CO(2)/HCO(3)(-) buffered inorganic plasma closely mimics physiological conditions.
    • This new system provides a more accurate model for studying ceramic biomineralization.
    • It offers a superior alternative to traditional SBF for biomimetic mineralization studies.