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A simple method for the construction of small format tissue arrays.
A Hidalgo1, P Piña, G Guerrero
1Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Centro Médico Nacional Siglo XXI-IMSS, México DF, 06720.
Journal of Clinical Pathology
|February 1, 2003
Summary
This study presents an affordable method for creating 36-sample tissue arrays using common laboratory materials. This technique allows for parallel molecular target evaluation, making it accessible for smaller labs.
Area of Science:
- Biotechnology
- Oncology Research
- Molecular Pathology
Background:
- Tissue arrays enable high-throughput molecular target evaluation.
- Commercial tissue arrayers are costly, limiting accessibility for smaller laboratories.
- Developing cost-effective tissue array construction methods is crucial for broader research applications.
Purpose of the Study:
- To describe a novel, low-cost method for constructing 36-sample tissue arrays.
- To demonstrate the utility of self-made tissue arrays for molecular target detection.
- To provide an accessible alternative to expensive commercial tissue arrayers.
Main Methods:
- Utilized a blunted 16-gauge needle to extract paraffin wax cylinders.
- Manually created a 6x6 matrix on a blank paraffin wax block.
- Injected tissue cores from cervical carcinoma samples into the matrix using a 14-gauge needle.
Main Results:
- Successfully constructed a 36-sample tissue array using readily available materials.
- Sectioned the array and performed immunodetection of CD44 variant 9 and interleukin 18.
- Achieved satisfactory results in molecular target detection, validating the method's efficacy.
Conclusions:
- The described method offers a cost-effective and accessible approach to tissue array construction.
- This technique empowers smaller laboratories to conduct high-throughput molecular analyses.
- The self-made tissue arrays are suitable for evaluating molecular targets like CD44 variant 9 and interleukin 18.