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Baculovirus studies in new, indigenous lepidopteran cell lines
U Pant1, A B Sudeep, S S Athawale
1National Institute of Virology, 20-A, Dr. Ambedkar Road, Pune 411 001, India.
Indian Journal of Experimental Biology
|February 4, 2003
Summary
Newly established lepidopteran cell lines show high susceptibility to specific baculoviruses, enabling in vitro production of bio-insecticides and recombinant proteins for diagnostics and vaccines.
Area of Science:
- Entomology
- Virology
- Cell Biology
Background:
- Establishment of novel lepidopteran cell lines is crucial for insect virus research.
- Understanding cell line susceptibility to baculoviruses is key for developing biological control agents and expression systems.
Purpose of the Study:
- To establish and characterize lepidopteran cell lines.
- To determine the susceptibility of these cell lines to various baculoviruses.
- To evaluate the potential applications of these cell lines in biotechnology.
Main Methods:
- Eight lepidopteran cell lines were established from different tissues and developmental stages.
- Susceptibility assays were performed using Spodoptera litura nuclear polyhedrosis virus (SLNPV) and Helicoverpa armigera NPV (HaNPV).
- Cryo-preserved cell lines were tested for baculovirus susceptibility.
Main Results:
- Two Spodoptera litura cell lines were highly susceptible to SLNPV, and one Helicoverpa armigera cell line was highly susceptible to HaNPV.
- In vitro-produced SLNPV and HaNPV caused 100% larval mortality.
- Cryo-preservation did not significantly alter cell line susceptibility to baculoviruses.
- Cell lines also showed susceptibility to Autographa californica NPV and Bombyx mori NPV.
Conclusions:
- Established lepidopteran cell lines are valuable tools for in vitro baculovirus production, serving as bio-insecticides.
- These cell lines are suitable for baculovirus expression vector studies, facilitating recombinant protein production for diagnostic kits and vaccines.