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Updated: Aug 19, 2026
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
[Changes in transforming growth factor beta 1 mRNA and its clinical value in patients with schistosomal liver
1Institute of Infectious Diseases, Zhejiang Medical University, Hangzhou 310003.
Aim:
To explore the changes in TGF-beta 1 mRNA in patients with schistosomal liver fibrosis.
Methods:
Reverse-transcription polymerase chain reaction and dot blot analysis were used to determine the level of TGF-beta 1 mRNA in PBMC. The serum levels of hyaluronic acid, type IV collagen, laminine and procollagen III peptide were also measured simultaneously.
Results:
The mean level (m +/- s) of PBMC TGF-beta 1 mRNA was significantly higher in late-stage schistosomiasis group (LSS) (1.26 +/- 0.14), liver cirrhosis group (LC) (2.05 +/- 0.81) and hepatocellular carcinoma group (HCC) than that in control group (0.62 +/- 0.40) (P < 0.05). PBMC TGF-beta 1 mRNA level was higher in LC and HCC than that in LSS (P < 0.05). No significant difference was found in TGF-beta 1 mRNA between PBMC and liver tissue in patients with HCC. All patients with increased fibrogenic activity (i.e., abnormal levels of serum HA, Col-IV and LN) had increased levels of TGF-beta 1 mRNA.
Conclusion:
The level of TGF-beta 1 mRNA is correlated with the fibrotic activity of liver, the increasement of TGF-beta 1 mRNA in schistosomiasis patients after praziquantel treatment might induce liver fibrosis.
