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Related Experiment Videos

Creatinine assays: time for action?

Nigel Lawson1, Tim Lang, Arthur Broughton

  • 1Department of Clinical Chemistry, City Hospital, Nottingham NG5 1PB, UK. nlawson@ncht.trent.nhs.uk

Annals of Clinical Biochemistry
|February 5, 2003
PubMed
Summary
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Laboratory creatinine measurements show significant variation between methods, impacting accuracy. Standardization is crucial to minimize discrepancies in plasma creatinine results and improve patient care.

Area of Science:

  • Clinical Chemistry
  • Laboratory Medicine
  • Analytical Chemistry

Background:

  • Significant variations in plasma creatinine results across laboratories were observed.
  • Inaccuracies were noted when using commercial lyophilized standards for creatinine estimation.

Purpose of the Study:

  • To assess the variation and accuracy of different plasma creatinine measurement methods.
  • To identify discrepancies in creatinine testing across multiple laboratories.

Main Methods:

  • Plasma creatinine was measured in 47 samples using four different methods across nine laboratories.
  • Tandem mass spectrometry (MS) was used to determine the accuracy of the methods.
  • Commercial authenticated samples were analyzed by all participating laboratories.

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Main Results:

  • All four methods yielded significantly different plasma creatinine results (P<0.0001).
  • Results varied by up to 33% between methods, with Ortho method generally producing higher values.
  • Discrepancies were observed when comparing method results to tandem MS, particularly concerning matrix effects with lyophilized standards.

Conclusions:

  • No single method demonstrated good agreement with tandem MS, highlighting significant inter-method variability (up to 30%).
  • Standardization of plasma creatinine measurement across laboratories is recommended to reduce errors and improve reliability.