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[Study on inducing an artemisinin-resistant line of Plasmodium berghei]
Ying-dan Chen1, Bao-ying Lin, Jia-xun Zhang
1Institute for Parasitic Disease Control and Prevention, Chinese Center for Disease Control and Prenention, Shanghai 200025.
Objective:
To induce a line of Plasmodium berghei with resistance to artemisinin.
Methods:
The major methods included blood transmission from passage to passage and progressive increase of drug pressure.
Results:
The resistant lines were developed by different protocols: (A) The initial dosage of artemisinin was 126.2 mg/kg which was increased by 60 mg/kg for the next passage and boosted by 126.2 mg/kg for every other passage. As developed to passage 60 and 76, the resistant index was 18.39:1 and 14.89:1 respectively, then decreased gradually. For passage 108, the dosage was 8,862.5 mg/kg, but the resistant index was only 10.49:1. (B) Using passage 66 from (A) as the source, a dosage of 4,000 mg/kg was given each week, the resistance of the passage 40 increased significantly with an index of 27.5:1. (C) Using passage 19 of (B) as the source, drug was administered at the dose of 2,000 mg/kg each week. The resistant index of passage 15 was 17.41:1.
Conclusion:
Line of P. berghei with medium level resistance to artemisinin was established.