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[Construction and characterization of the cDNA library from Schistosoma japonicum cercariae]

Xiao-guang Chen1, Hua Li, Hong-juan Peng

  • 1Department of Parasitology, Institute of Tropical Medicine, First Military Medical University, Guangzhou 510515.

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases
|February 6, 2003
PubMed
Abstract

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
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