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[Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal
1Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai 200025.
Objective:
To detect circulating antigen in the patients with schistosomiasis.
Methods:
Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA.
Results:
Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections.
Conclusion:
PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.

