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Updated: Sep 27, 2026
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Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
[Improvement in the preparation of Paragonimus westermani chromosome for karyotype analysis]
Shao-hong Chen1, Zheng-shan Chang, Ming-gang Chen
1Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025.
Abstract
Objective:
To improve Paragonimus westermani chromosome preparation technique.
Methods:
After being exposed to colchicine, the gonadal cells of P. westermani were treated by the following procedures: hypotonicity, fixation, dropping onto a slide and staining.
Results:
The chromosome number of P. westermani is 22, and the karyotype is 2m + 6Sm + 14t chromosome.
Conclusion:
The improved technique of chromosome preparation is feasible to operate and the chromosome is clear enough for karyotype analysis.
