Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modeling O2 transport within engineered hepatic devices.

Randall E McClelland1, Jeffrey M MacDonald, Robin N Coger

  • 1Department of Mechanical Engineering, University of North Carolina at Charlotte, Charlotte, North Carolina, USA. rncoger@uncc.edu

Biotechnology and Bioengineering
|February 6, 2003
PubMed
Summary

This study developed a computational model to predict oxygen transport in bioartificial liver (BAL) devices. The model shows that enhancing extracellular matrix (ECM) with porous, convective flows significantly improves oxygen delivery to hepatocytes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequential Penta-Omic Extraction Method Using Single Biospecimens of Post-mortem Human Brain.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Nano-fibre Integrated Microcapsules: A Nano-in-Micro Platform for 3D Cell Culture.

Scientific reports·2023
Same author

Direct Detection of Glutathione Biosynthesis, Conjugation, Depletion and Recovery in Intact Hepatoma Cells.

International journal of molecular sciences·2022
Same author

Green Chemistry Preservation and Extraction of Biospecimens for Multi-omic Analyses.

Methods in molecular biology (Clifton, N.J.)·2022
Same author

A Unidirectional 96-Well Fluidic Culture Platform for Upstream Cell Dosing with Subsequent Downstream Nonlinear and Ascending Exposure Gradients for Real-Time and Cell-Based Toxicity Screening Environments.

Applied in vitro toxicology·2022
Same author

Author Correction: Combination of ERK and autophagy inhibition as a treatment approach for pancreatic cancer.

Nature medicine·2020

Area of Science:

  • Biomedical Engineering
  • Biomaterials Science
  • Computational Modeling

Background:

  • Oxygen transport is critical for hepatocyte viability in bioartificial liver (BAL) devices.
  • Existing BAL designs face challenges in efficient oxygen supply.
  • Computational models can predict outcomes and guide design improvements, reducing experimental costs.

Purpose of the Study:

  • To develop and validate a computational model for oxygen (O2) transport in various BAL designs.
  • To analyze the impact of extracellular matrix (ECM) modifications on O2 distribution.
  • To evaluate an enhancement technique for improving O2 transport through porous ECMs.

Main Methods:

  • A computational model simulating O2 transport through collagen and microporous collagen ECMs was developed.

Related Experiment Videos

  • The model analyzed hollow fiber (HF), flat plate (FP), and spheroid BAL configurations.
  • O2 transport was predicted by modifying boundary conditions, consumption levels, ECM permeability, and void fractions.

    • Main Results:

    • The computational model's accuracy was validated against experimental data for the HF BAL system.
    • Oxygen transport in all analyzed BAL designs was significantly improved by incorporating porous ECMs with diffusive and convective flows.
    • The hollow fiber design demonstrated superior O2 transport control due to convective flow on both surfaces.

    Conclusions:

    • The developed computational model accurately predicts O2 transport in BAL devices.
    • Enhancing ECM with porous structures and convective flow is an effective strategy to improve hepatocyte oxygenation.
    • The HF BAL design offers greater control over O2 transport compared to FP and spheroid designs.