Related Experiment Videos
[Tissue specificity and expression of a human sperm protein's gene BSD-2.4]
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Abstract
Objective:
To investigate the tissue specificity and expression in spermatogenic stage of BSD-2.4 cDNA which might be related with fertility and its sequence was received by GenBank.
Methods:
Dot blot, Northern blot, and in situ hybridization were used.
Results:
Northern blot analysis of sixteen human tissues showed that BSD-2.4 cDNA had one transcript only with testis. BSD-2.4 mRNA was detected in germ cells of human testis.
Conclusions:
BSD-2.4 is a testis-specific protein. The cDNA was expressed throughout the stages of the spermatogenic cycle.