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Published on: December 21, 2011
[Relationship between complement activation and reactive oxygen species generation in inflammatory response]
1Department of Pathology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005.
Objective:
To verify the proposed activation feedback mechanism between complement and polymorphonuclear leukocytes(PMN) derived reactive oxygen species(ROS).
Methods:
In vitro tests were designed to identify the activation feedback process by alternative activations of complement by inulin or PMNs by 12-O-tetradecanoylphorbol-13-acetate(TPA) in the test medium in consisting serum complement and isolated PMNs, with chemiluminescence technique to determine the production of ROS and complement fixation trial for complement activations.
Results:
Evidently that activated complement could activate PMNs to release ROS which further activated complement, so the activating feedback cycle was set.
Conclusions:
The complement and PMNs-ROS activation feedback cycle mechanism was recognized. It was expected to serve for delineating the regulation of inflammatory response.
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