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[Cloning and expression of bluetongue virus VP3 protein in insect cells]
1Institute of Virology, Chinese Academy of Preventive Medicine, Beijing 100052.
Objective:
To elucidate the biological activities of VP3 protein of bluetongue virus (BTV) expressed in insect cells and to assemble, in future, the BTV core-like particles.
Methods:
First, full length of BTV13 L3 cDNA was constructed from 2 sequenced clones. The silver staining of the splicing region proved that the construction was correct. Then, a recombinant baculovirus expression vector pFB1BL3 was constructed to express the L3 gene in insect cells.
Results:
BTV13 VP3 could be highly expressed in Sf-9 cells, the production could occupy up to 10%-15% of the total cell protein. Core-like particles could be observed after co-expression of the VP3 and VP7 in insect cells.
Conclusion:
VP3 of BTV expressed in insect cells possesses biological activity and can be used to assemble BTV core-like particles.