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[Cloning and sequence analysis of 55 K protein of egg drop syndrome virus]
1State Key Laboratory of Molecular Virology and Genetic Engineering, CAMP, Beijing 100052.
Objective:
For understanding the characteristics of genomic structure of egg drop syndrome virus(EDSV).
Methods:
Nucleic acid was extracted using routine method from weak virulent strain AA-2 of EDSV isolated from Chinese sick hens. Construction of the whole genomic library was by hydrolysis with Hind III, strand encoding 55 K gene locating in Hind III--A segment was sequenced and analyzed.
Results:
The open reading frame has a length of 1,014 nt and codes a polypeptide of 337 amino acids with molecular weight of 38,200. Analysis of the amino acid sequence revealed a homology from 25.5%-32.4% to the 55 K protein of human adenovirus types 2, 12, 40, canine adenovirus and fowl adenoviruses of group 1, whereas to ovine adenovirus is 46.4%.
Conclusion:
The genomic structure of EDSV has some relationship with adenoviruses.
