Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Purification strategies for combinatorial and parallel chemistry.

Paul J Edwards1

  • 1Discovery Chemistry, Pfizer Global Research and Development, Ramsgate Road, Sandwich, Kent, CT13 9NJ, UK. paul_edwards@sandwich.pfizer.com

Combinatorial Chemistry & High Throughput Screening
|February 7, 2003
PubMed
Summary

This review covers purification techniques for parallel and combinatorial chemistry, evaluating methods like polymer-assisted purification and chromatography. It highlights the strengths and weaknesses of each approach for compound purification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The creation and characterisation of a National Compound Collection: the Royal Society of Chemistry pilot.

Chemical science·2018
Same author

Identification of potent and orally bioavailable nucleotide competing reverse transcriptase inhibitors: in vitro and in vivo optimization of a series of benzofurano[3,2-d]pyrimidin-2-one derived inhibitors.

Bioorganic & medicinal chemistry letters·2013
Same author

Nucleotide competing reverse transcriptase inhibitors: discovery of a series of non-basic benzofurano[3,2-d]pyrimidin-2-one derived inhibitors.

Bioorganic & medicinal chemistry letters·2013
Same author

Identification of benzofurano[3,2-d]pyrimidin-2-ones, a new series of HIV-1 nucleotide-competing reverse transcriptase inhibitors.

Bioorganic & medicinal chemistry letters·2013
Same author

Discovery of a series of imidazopyrazine small molecule inhibitors of the kinase MAPKAPK5, that show activity using in vitro and in vivo models of rheumatoid arthritis.

Bioorganic & medicinal chemistry letters·2012
Same author

Assessing atropisomer axial chirality in drug discovery and development.

Journal of medicinal chemistry·2011

Area of Science:

  • Organic Chemistry
  • Analytical Chemistry
  • Process Chemistry

Background:

  • Parallel and combinatorial chemistry enable rapid synthesis of compound libraries.
  • Efficient purification is crucial for isolating desired intermediates and final products.
  • Existing purification methods present challenges in terms of scalability and efficiency.

Purpose of the Study:

  • To provide a comprehensive review of purification methods applicable to parallel and combinatorial chemistry.
  • To critically evaluate the advantages and disadvantages of various purification techniques.
  • To guide researchers in selecting appropriate purification strategies.

Main Methods:

  • Review of polymer-assisted purification.
  • Analysis of liquid-phase combinatorial chemistry purification.

Related Experiment Videos

  • Survey of fluorous synthesis purification strategies.
  • Evaluation of liquid-liquid and solid-phase extraction.
  • Assessment of reverse-phase High-Performance Liquid Chromatography (HPLC).
  • Examination of supercritical fluid chromatography (SFC).

    • Main Results:

    • Each method's strengths and weaknesses are critically assessed.
    • Polymer-assisted purification offers advantages in handling and recovery.
    • Chromatographic techniques like HPLC and SFC provide high resolution but can be resource-intensive.
    • Extraction methods are versatile but may require optimization for specific compounds.

    Conclusions:

    • The choice of purification method depends on the specific chemical context and scale.
    • A combination of techniques may be necessary for optimal purification.
    • Further development is needed to enhance the efficiency and scalability of purification processes in combinatorial chemistry.