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[Construction of multivalent DNA vaccine against Schistosoma japonicum]
1Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030.
Objective:
To construct a multivalent DNA vaccine.
Methods:
The multivalent DNA vaccine candidates pBK-Sj26-Sj23, pBK-Sj32-Sj23 were constructed based on the plasmids pBluescript-Sj26, pBluescript-Sj32 and pBluescript-Sj23 with three pairs of specific primers using DNA recombinant technique. In the primers, a synthetic linker sequence encoding a peptide was designed, and the antigen genes Sj26 and Sj23, Sj32 and Sj23 were then ligated. After identification, the quadriceps muscle of mice were immunized with the multivalent antigen genes. Four weeks after immunization, the multivalent antigen genes were present in the muscular tissue of mice by PCR.
Results:
The eukaryotic plasmids including multivalent antigens of S. japonicum were constructed successfully, and the plasmids including multivalent antigen gene could be stably existing in the muscle tissue of mice and the multivalent antigens could be expressed in the muscle tissue cells of mice.
Conclusion:
A multivalent S. japonicum DNA vaccine has been established.