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Updated: Sep 27, 2026

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Published on: March 12, 2015
[Exogenous nitric oxide induces apoptosis in tachyzoites of Toxoplasma gondii]
Objective:
To explore whether NO is able to induce apoptosis in Toxoplasma gondii tachyzoites.
Methods:
Apoptosis induced by NO in T. gondii tachyzoites was investigated by TUNEL (terminal-deoxynucleotidyl transferase mediated d-UTP nick end labeling) method, electron microscopy and agarose gel electrophoresis.
Results:
NO donor, sodium nitroprusside (SNP), was found to induce apoptosis in Toxoplasma gondii tachyzoites in a time- and dose-dependent manner by TUNEL detection. N-acetylcysteine, a NO scavenger, could inhibit SNP-induced apoptosis in the tachyzoites while potassium ferricyanide could not induce apoptosis in the tachyzoite. Electron macroscopy showed that SNP-treated tachyzoites possessed typical morphological features of apoptosis, including chromatin condensation below the nuclear membrane, nuclear pyknosis, and formation of apoptotic body. Agarose gel electrophoresis revealed that SNP-treated tachyzoite DNA fragment exhibited characteristic "DNA ladder" after 15 to 20 h.
Conclusion:
SNP, NO donor, might induce apoptosis in T. gondii tachyzoites in terms of characteristic morphological and biochemical features.
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