Related Experiment Videos
[Immunostimulatory activity elicited by CpG sequences in Cysticercus cellulosae paramyosin cDNA]
1Department of Medical Genetic, Second Military Medical University, Shanghai 200433.
Objective:
To analyse the immunostimulatory activity of CpG sequences in cysticercus cellulosae paramyosin (also named Antigen B, AgB) cDNA.
Methods:
C57BL/6 mice were immunized with pcDNA3-AgB plasmid, pcDNA3-AgB' (CpG sequences were mutated), pcDNA3 or AgB protein and two weeks later, immune response was assayed by ELISA.
Results:
IgG and IgG2a were detectable at week 2 after immunization and continually increased until week 4. The antibody levels elicited by pcDNA3-AgB were significantly higher(P < 0.05) than those elicited by others.
Conclusion:
After pcDNA3-AgB plasmid inoculation, the immune response of mouse was elicited not only by the AgB protein but also by the CpG immunostimulatory sequences in the AgB cDNA.