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[Detection of anti-Spirometra erinaceieuropaei antibody using ELISA]
1Department of Epidemiology, Hebei Medical University, Shijiazhuang 050017.
Abstract
Objective:
To study specific diagnosis of Spirometra erinaceieuropaei.
Methods:
An enzyme-linked immunosorbent assay (ELISA) was studied using highly pure gene engineering antigen expressed by the recombination of the cloned cysteine proteinase gene of Spirametra erinaceieuropaei with expression vector pMAL-c2. Six sera from patient infected with Spirometra erinaceieuropaei were detected using this method.
Results And Conclusion:
The results showed that the gene engineering antigen reacted strongly with the sera from Spirometra erinaceieuropaei-infected patients, but did not with the sera from Cysticercus cellulosae-infected patients.