Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vaginal lactobacilli: self- and co-aggregating ability.

Virginia S Ocaña1, María Elena Nader-Macías

  • 1CERELA (Centro de Referencia para Lactobacilos)-CONICET, Chacabuco 145, 4000 Tucumán, Argentina.

British Journal of Biomedical Science
|February 8, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Draft genome sequence of Enterococcus mundtii CRL1656.

Journal of bacteriology·2011
Same author

Stability of lactobacilli encapsulated in various microbial polymers.

Journal of bioscience and bioengineering·2011
Same author

Lactic acid bacteria isolated from young calves--characterization and potential as probiotics.

Research in veterinary science·2011
Same author

Characterization of salivaricin CRL 1328, a two-peptide bacteriocin produced by Lactobacillus salivarius CRL 1328 isolated from the human vagina.

Research in microbiology·2009
Same author

Stability of freeze-dried vaginal Lactobacillus strains in the presence of different lyoprotectors.

Canadian journal of microbiology·2009
Same author

Influence of vitamins and osmolites on growth and bacteriocin production by Lactobacillus salivarius CRL 1328 in a chemically defined medium.

Canadian journal of microbiology·2009
Same journal

Artificial intelligence approaches in biological age prediction: current status and challenges.

British journal of biomedical science·2026
Same journal

Challenges and emerging strategies for genome-wide evaluation of loss of imprinting in cancer.

British journal of biomedical science·2026
Same journal

Application of non-invasive preimplantation genetic screening for aneuploidy through spent embryo culture media analysis at 48 and 54 hours after embryo cleavage.

British journal of biomedical science·2026
Same journal

Training on PD-L1 scoring in non-small cell lung cancer with high intra- and inter-reader agreement: results of a worldwide microscopic/digital image-based training of 751 pathologists.

British journal of biomedical science·2026
Same journal

Performance of a quality control center supporting national antimicrobial resistance surveillance.

British journal of biomedical science·2026
Same journal

The Molecular Pathology of Non-Malignant Haematological Disease.

British journal of biomedical science·2026
See all related articles

Vaginal lactobacilli use surface peptides for self-aggregation and co-aggregation with Candida species, a key defense mechanism. Mannose specifically inhibits this interaction, highlighting its role in maintaining vaginal health.

Area of Science:

  • Microbiology
  • Vaginal microbiome research
  • Bacterial adhesion mechanisms

Background:

  • Lactic acid bacteria, particularly Lactobacillus species, dominate the healthy vaginal tract.
  • These bacteria form a protective barrier against pathogens through adhesion, self-aggregation, and co-aggregation.
  • Understanding these aggregation mechanisms is crucial for comprehending vaginal health and preventing infections.

Purpose of the Study:

  • To investigate the factors responsible for the self-aggregating ability of vaginal lactobacilli.
  • To identify the mechanisms underlying the co-aggregation of vaginal lactobacilli with Candida species.
  • To elucidate the molecular interactions involved in these aggregation processes.

Main Methods:

  • Quantitative assessment of self-aggregation and co-aggregation using spectrophotometry (decrease in absorbance).

Related Experiment Videos

  • Qualitative analysis of microbial aggregation patterns via light microscopy.
  • Enzymatic sensitivity tests (trypsin, pepsin) to characterize the nature of aggregating factors.
  • Inhibition assays using various carbohydrates, including mannose, to identify receptor-ligand interactions.

    • Main Results:

    • Self-aggregation in most tested vaginal lactobacilli was mediated by trypsin-sensitive peptides/proteins.
    • A specific strain, Lactobacillus acidophilus CRL 1294, exhibited trypsin-resistant, pepsin-sensitive self-aggregation.
    • Lactobacillus acidophilus CRL 1294 and Lactobacillus salivarius CRL 1328 demonstrated co-aggregation with Candida spp.
    • Co-aggregation involved bacterial surface peptides and yeast surface carbohydrates, with mannose effectively inhibiting the interaction.

    Conclusions:

    • Vaginal lactobacilli employ surface-associated peptides for self-aggregation and co-aggregation with Candida species.
    • The interaction between lactobacilli and Candida is specific, involving mannose-sensitive carbohydrate-peptide recognition.
    • These aggregation mechanisms are vital components of the protective barrier function of the vaginal microbiome.