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The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Lactic acid bacteria isolated from young calves--characterization and potential as probiotics
Natalia C Maldonado1, Clara Silva de Ruiz, María Claudia Otero
1Centro de Referencia para Lactobacilos (CERELA-CONICET), Chacabuco 145, 4000 San Miguel de Tucumán, Argentina.
Research in Veterinary Science
|April 19, 2011
Summary
Researchers identified specific lactic acid bacteria (LAB) strains from calves with probiotic potential. These findings aid in developing criteria for selecting effective multi-strain probiotic products for animal health.
Area of Science:
- Microbiology
- Animal Science
- Probiotics
Background:
- Lactic acid bacteria (LAB) are crucial probiotics for maintaining mucosal balance in humans and animals.
- Newborn calves are vulnerable to various health issues, necessitating effective probiotic interventions.
- Current criteria for selecting probiotic strains are insufficient for targeted product development.
Purpose of the Study:
- To isolate and characterize lactic acid bacteria (LAB) from calves.
- To evaluate the potential probiotic properties of these LAB isolates.
- To establish criteria for selecting LAB strains for multi-strain probiotic products.
Main Methods:
- Isolated 96 LAB strains from calf feces and oral cavities.
- Screened isolates for surface properties, hydrogen peroxide production, pathogen inhibition, bacteriocin production, and tolerance to low pH and high bile concentrations.
- Genetically identified promising strains, assessed their compatibility, and evaluated exopolysaccharide production.
Main Results:
- Identified a subset of LAB isolates with desirable probiotic properties, including hydrophobicity, autoagglutination, pathogen inhibition, and bacteriocin production.
- Some strains demonstrated tolerance to challenging conditions like low pH and high bile.
- Genetic identification and compatibility assays were performed on selected strains.
Conclusions:
- The study provides valuable data for establishing selection criteria for probiotic LAB strains.
- Findings support the development of a multi-strain probiotic product for calves.
- Further in vivo assays are recommended to validate the efficacy of selected strains.
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