Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Prophage-dependent plasmid integration in Staphylococcus aureus.

M D Schwesinger, R P Novick

    Journal of Bacteriology
    |August 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The complete genomes of Staphylococcus aureus bacteriophages 80 and 80α--implications for the specificity of SaPI mobilization.

    Virology·2010
    Same author

    Climate change and the integrity of science.

    Science (New York, N.Y.)·2010
    Same author

    Role of the double-strand origin cruciform in pT181 replication.

    Plasmid·2001
    Same author

    Molecular genetics of SaPI1--a mobile pathogenicity island in Staphylococcus aureus.

    Molecular microbiology·2001
    Same author

    Pathogenicity and resistance islands of staphylococci.

    Microbes and infection·2001
    Same author

    Subinhibitory clindamycin differentially inhibits transcription of exoprotein genes in Staphylococcus aureus.

    Infection and immunity·2001
    Same journal

    The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

    Journal of bacteriology·2026
    Same journal

    Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

    Journal of bacteriology·2026
    Same journal

    Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

    Journal of bacteriology·2026
    Same journal

    DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

    Journal of bacteriology·2026
    Same journal

    Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

    Journal of bacteriology·2026
    Same journal

    Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

    Journal of bacteriology·2026
    See all related articles

    Reversion of thermosensitive, replication-defective (TSR) mutant penicillinase plasmids occurs via back mutation, suppression, or integration. Phage phi 11 significantly enhances integration frequency, affecting plasmid-linked resistances and stability.

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • Thermosensitive, replication-defective (TSR) mutant penicillinase plasmids exhibit temperature-dependent instability.
    • Understanding plasmid reversion mechanisms is crucial for genetic engineering and stability studies.

    Purpose of the Study:

    • To investigate the reversion pathways of TSR mutant penicillinase plasmids.
    • To characterize the role of phage phi 11 in plasmid integration and reversion.

    Main Methods:

    • Induction of reversion in TSR mutant penicillinase plasmids.
    • Analysis of reversion products: back mutation, suppression, and integration.
    • Characterization of integrated plasmid-prophage complexes using temperature stability and growth assays.
    • Molecular analysis of plasmid and prophage DNA.

    Related Experiment Videos

  • Transduction experiments to assess reversion and integration stability.

    • Main Results:

    • All three expected reversion classes (back mutation, suppression, integration) were observed.
    • Phage phi 11 presence increased integration frequency by approximately 10^3-fold.
    • Prophage-dependent integration inactivated plasmid-linked resistances but was reversible upon transduction.
    • Integrated plasmid-prophage complexes showed temperature-dependent stability, being stable at 43°C but unstable at 32°C.
    • Integration also occurred without prophage, retaining resistances and showing stability at all temperatures.
    • Excision complexes, potentially plasmid-phage co-integrates, were observed.

    Conclusions:

    • The prophage (phi 11) promotes plasmid integration, likely at or near its attachment site.
    • Plasmid-prophage integration impacts plasmid stability and function, with reversibility observed.
    • Integration in the absence of prophage results in stable, resistant, and heritable integrated states.