[Study on lipids and other volatile constituents in Pheretima aspergillum]

To study chemical constituents in Pheretima aspergillum, three kinds of fractions were obtained from this drug by soxhlet extraction with different solvents, and the chemical structures of thirty-six volatile components were identified by means of GC-MS. The eleven in ether fraction were all lipids and the relative content of non-saturated fatty acid was the highest(27.70%) such as oleic acid, linoleic acid, arachidonic acid and eicosatrienoic acid; There were eight lipids in acetone fraction (35.75%), which included one kind of nonsaturated fatty acid (linoleic acid); There were thirteen lipids in ethanol fraction (72.09%), which non-saturated fatty acid has never been detected. This study has determined the lipid composition in Pheretima aspergillum, especially non-saturated fatty acid, and afforded chemical base to cardio-cerebro-vascular therapy.