[Determination of the saflor yellow-A in Carthamin tinctorius]

The content of the saflor yellow-A in Carthamin tinctorius medicinal materials was determined by the HPLC method. The C18-ODS(150 x 4.5 mm) column and methanol-acetonitrile-0.7% H3PO4(26:2:72) as a mobile phase were used. The flow rate was 1.0 ml/min and the detective wavelength was 403 nm. The average recovery was 100.72% and the relative standard deviation (RSD) was 1.73%.