Related Experiment Video
Updated: Sep 21, 2026
Identification of OTX1 and OTX2 As Two Possible Molecular Markers for Sinonasal Carcinomas and Olfactory Neuroblastomas
Published on: February 28, 2019
[Detection of telomerase activity in salivary tumor and its significance]
Liang-jian Chen1, Jian-hua Huang, Zan Li
1Department of Stomatology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
Objective:
To investigate telomerase activity in human salivary cancer and corresponding adjacent tissues and to explore the possibility of telomerase as a tumor marker and its clinical significance.
Methods:
Twenty-eight salivary cancers, 28 adjacent peritumoral tissues, 10 mixed tumors, 6 adenolymphomas, and 5 normal salivary tissues were examined for telomerase activity by the silver-staining TRAP assay based on PCR.
Results:
Twenty-five of the 28 salivary cancers and 2 of the 28 adjacent peritumoral tissue specimens were positive for telomerase activity with a positive rate of 89.3% and 6.3%, respectively. Telomerase activity was negative in the 10 mixed tumors, 6 adenolymphomas, and 5 normal salivary tissues. There was no correlation between the clinical stage of salivary cancer and its expression of telomerase activity (P > 0.05).
Conclusion:
Positive telomerase activity occurs in the majority of salivary cancers examined. It can be used as a tumor marker in the diagnosis of salivary cancer. Detection of telomerase activity in the adjacent peritumoral tissues can be used as a monitoring marker after treatment.
More Related Videos
08:26Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Published on: June 12, 2018
06:38Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
Published on: May 3, 2019
