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[Association of platelet endothelial cell adhesion molecule-1 gene polymorphism with coronary heart disease]
Fu-chun Song1, Ai-hua Chen, Xiao-ming Tang
1Department of Cardiology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. chenaha@21cn.com
Insights
Platelet endothelial cell adhesion molecule-1 (PECAM-1) gene polymorphisms are associated with coronary heart disease (CHD). Specific variants, Leu125Val and Ser563Asn, show significant differences in allele frequencies and genotype distributions in CHD patients.
Area of Science:
- Genetics
- Cardiology
- Molecular Biology
Background:
- Coronary heart disease (CHD) is a leading cause of mortality worldwide.
- Genetic factors play a role in CHD susceptibility.
- Platelet endothelial cell adhesion molecule-1 (PECAM-1) is involved in vascular function and inflammation.
Purpose of the Study:
- To investigate the association between specific polymorphisms in the PECAM-1 gene (Leu125Val and Ser563Asn) and the risk of developing coronary heart disease.
- To determine if these genetic variations are independent risk factors for CHD.
Main Methods:
- Case-control study design involving 156 patients diagnosed with CHD and 75 healthy controls.
- Genotyping of PECAM-1 gene polymorphisms (Leu125Val and Ser563Asn) using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
- Statistical analysis of allele frequencies and genotype distributions between CHD and control groups.
Main Results:
- Significant differences were observed in allele frequencies and genotype distributions of the PECAM-1 gene between the CHD and control groups.
- The allele frequencies of 125Val and 563Asn, as well as the genotype distributions of 125Val/Val and 563Asn/Asn, showed particularly conspicuous differences (P<0.05).
Conclusions:
- PECAM-1 gene polymorphism, specifically the Leu125Val and Ser563Asn variants, may represent a genetic risk factor for coronary heart disease.
- These findings contribute to understanding the genetic basis of CHD susceptibility.
Objective:
To investigate the association of Leu125Val and Ser563Asn polymorphism of the gene encoding platelet endothelial cell adhesion molecule-1(PECAM-1) with coronary heart disease.
Methods:
This study included 156 patients with the diagnoses of coronary heart disease (CHD) and coronary lesions derived from electrocardiography, myocardial enzyme analysis and coronary angiography as the CHD group, and another 75 in-patients admitted within the same period who showed no signs of CHD in the above examinations constituted the control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was employed to examine the missense polymorphism of PECAM-1gene in the position of Leu125Val and Ser563Asn.
Results:
There were significant differences between CHD and control group in terms of the allele frequencies and genotype distributions of PECAM-1 gene, and the differences were especially conspicuous in the allele frequencies of 125Val and 563Asn (P<0.05) and genotype distributions of 125Val/Val and 563Asn/Asn.
Conclusion:
PECAM-1 gene polymorphism 1 may be a genetic risk factor for coronary heart disease.