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[Study on enrichment process of Cornus officinalis glycosides with macroporous resin and active carbon]
Wenxia Pi1, Baochang Cai, Yang Pan
1Nanjing University of Traditional Chinese Medicine, Nanjing 210029.
Objective:
To study the technological parameters of the enrichment purification of Cornus officinalis glycosides (COG) with macroporous resin and active carbon.
Methods:
COG was isolated and purified by active carbon and macroporous resin HPD100. The enrichment of COG was evaluated by HPLC method.
Results:
The optimum process was that 10 ml extract of Fructus Corni was absorbed with columns of macroporous resin and active carbon(R25 mm x H300 mm) and the columns were washed with 600 ml distilled water, then the morroniside and loganin was eluted from the macroporous resin and active carbon with ethanol.
Conclusion:
The elutive rate of morroniside and logann was about 85% by the macroporous resin, 96% by the active carbon. So this method enriching COG from Fructus Corni is practical.