[Isolation, purification and analysis of polysaccharides from Adenophora potaninii Korsh]

Polysaccharides, AP-1 and AP-3, were obtained through extraction of the roots of Adenophora potaninii Korsh by hot water, followed by the isolation and purification by DEAE-cellulose and Sephadex G-200 column chromatography. It was proved to be homogeneous by gel filtration chromatography and their average MW was estimated to be 8.3 x 10(4) and 6.3 x 10(4) respectively. Analysis of sugar composition showed that AP-1 is D-glucan, while AP-3 is composed of glucose, rhamnose, arabinose and glucronic acid in the molar ratio of 5:1:1:3.