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[Mechanism of apoptosis induced by squamocin in leukemia cells]
1Cancer Institute, Cancer Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. xfzhu70@yahoo.com
Aim:
To investigate the mechanism of apoptosis of HL60 cells induced by the annonaceous acetogenin, squamocin.
Methods:
Induction of apoptosis was determined through Hoechst33258 dye staining and DNA agarose gel electrophoresis. Expression of the proteins was detected using Western blot analysis. Caspase-3 activity was detected using caspase-3 kit.
Results:
Treatment of HL-60 cells with squamocin resulted in extensive nuclear condensation, DNA fragmentation, cleavage of the death substrate poly(ADP-ribose) polymerase (PARP) and induction of caspase-3 activity. Pretreatment of HL-60 cells with caspase-3 specific inhibitor DEVD-CHO prevented squamocin-induced DNA fragmentation, PARP cleavage and cell death. Stress-activated protein kinase (SAPK/JNK) was activated after treatment with squamocin in HL-60 cells.
Conclusion:
These results suggest that apoptosis of HL-60 cells induced by squamocin require caspase-3 activation, and could be related to SAPK activation.
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