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Updated: Sep 27, 2026

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
Chemometric discrimination of unfractionated plant extracts analyzed by electrospray mass spectrometry
Royston Goodacre1, Emma V York, James K Heald
1Institute of Biological Sciences, University of Wales Aberystwyth, Ceredigion SY23 3DA, UK.
Abstract:
Metabolic fingerprints were obtained from unfractionated Pharbitis nil leaf sap samples by direct infusion into an electrospray ionization mass spectrometer. Analyses took less than 30 s per sample and yielded complex mass spectra. Various chemometric methods, including discriminant function analysis and the machine-learning methods of artificial neural networks and genetic programming, could discriminate the metabolic fingerprints of plants subjected to different photoperiod treatments. This rapid automated analytical procedure could find use in a variety of phytochemical applications requiring high sample throughput.
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