Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Splicing regulation: the cell cycle connection.

Benjamin J Blencowe1

  • 1C.H. Best Institute, University of Toronto, 112 College Street, Room 410, Toronto, Ontario, Canada M5G 1L6. b.blencowe@utoronto.ca

Current Biology : CB
|February 21, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Alu-mediated RNA duplexes are associated with widespread exon skipping across primate transcriptomes.

Genome biology·2026
Same author

RNA-binding proteins TDP-43 and FUS promote R-loop resolution and regulate transcription termination.

The Journal of biological chemistry·2026
Same author

Splice isoform-perturbation coupled to single cell transcriptome profiling reveals functions of microexons in neurogenesis and autism-linked pathways.

Nature communications·2026
Same author

tRNA-overlapping long non-coding RNA loci repress codon-biased genes.

Cell reports·2025
Same author

No evidence that human GIGYF2 interacts with GRB10: implications for human disease.

Life science alliance·2025
Same author

A highly conserved neuronal microexon in DAAM1 controls actin dynamics, RHOA/ROCK signaling, and memory formation.

Nature communications·2025
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
Same journal

Tracking Satb2-positive retinal ganglion cells in zebrafish unveils developmental functional reorganization.

Current biology : CB·2026
Same journal

RhoGAP54D promotes cell size asymmetry and inhibits pulsatile myosin activity in Drosophila neural stem cells.

Current biology : CB·2026
Same journal

Increased rates of hybridization in swordtails are associated with water pollution.

Current biology : CB·2026
See all related articles

Gene repression during mitosis targets splicing. A new study shows dephosphorylation of splicing factor SRp38 causes mitotic repression, impacting gene expression.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Gene expression is tightly regulated during the cell cycle.
  • Mitosis involves significant cellular changes, including the repression of many genes.
  • Known mechanisms of mitotic repression involve post-translational modifications like phosphorylation of transcription, RNA processing, and translation factors.

Purpose of the Study:

  • To investigate whether splicing is also subject to repression during mitosis.
  • To identify the molecular mechanisms underlying potential splicing repression in mitosis.
  • To characterize the role of the newly identified splicing factor SRp38 in this process.

Main Methods:

  • Analysis of gene expression patterns during mitosis.
  • Biochemical assays to study protein phosphorylation states.

Related Experiment Videos

  • Identification and characterization of splicing factors involved in mitotic regulation.

    • Main Results:

    • Evidence suggests splicing is repressed during mitosis.
    • The splicing factor SRp38 was identified as a key player in mitotic repression.
    • Dephosphorylation of SRp38 was found to be the mechanism responsible for targeting splicing during mitosis.

    Conclusions:

    • Splicing is a regulated process during mitosis, contributing to overall gene expression control.
    • SRp38 acts as a critical mediator of splicing repression in mitosis through dephosphorylation.
    • Understanding SRp38's role provides new insights into the complex regulatory networks governing cell division and gene expression.