Crystallization and preliminary X-ray study of human dipeptidyl peptidase IV (DPPIV)
Related Experiment Video
Updated: Sep 27, 2026

Production, Crystallization and Structure Determination of C. difficile PPEP-1 via Microseeding and Zinc-SAD
Published on: December 30, 2016
Hajime Hiramatsu1, Kiyoshi Kyono, Hideaki Shima
1Discovery Research Laboratory, Tanabe Seiyaku Co. Ltd, 3-16-89 Kashima, Yodogawa-ku, Osaka 532-8505, Japan.
Abstract:
Human DPPIV has been expressed in the baculovirus system and purified and crystallized using the hanging-drop method. A crystal was obtained from 180 mM Gly-NaOH buffer pH 9.5 containing 18% PEG 4000 and 180 mM sodium acetate. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 118.04, b = 125.92, c = 136.84 A, and diffracts beyond 2.6 A resolution. There are two molecules per asymmetric unit, indicating a solvent content of 57.6%.
Related Concept Videos
Production of Pharmaceuticals
Dipeptidyl Peptidase 4 Inhibitors
X-ray Diffraction of Biological Samples
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal crystal...

