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Use of shark collagen for cell culture and zymography
Yoshihiro Nomura1, Nobuhide Kitazume
1Department of Applied Protein Chemistry, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan. ny318@cc.tuat.ac.jp
Abstract:
The uses of shark collagen as a matrix for cell culture and as a substrate for zymography were investigated. Fibroblasts were cultured on a gel matrix of shark type I collagen at 30 degrees C. The collagen gel had contracted by 4 days of incubation. Individual fibroblasts were visible against the transparent background of the contracted collagen as long, lean star-shaped cells. The matrix metalloproteinases (MMPs) from fibroblasts secreted from the medium more easily digested shark gelatin than pig gelatin. MMP-2, -9, and that of potential form were recognizable in the zymographic gel of shark gelatin.