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Design of a fluorescent electrophoretic mobility shift assay improved for the quantitative and multiple analysis of
Takuo Onizuka1, Sumiyo Endo, Masahiko Hirano
1Biological Sciences Department, Toray Research Center, Inc., 1111 Tebiro, Kamakura, Kanagawa 248-8555, Japan. Takuo_Onizuka@trc.toray.co.jp
Bioscience, Biotechnology, and Biochemistry
|February 25, 2003
Abstract:
We describe a protocol for the fluorescent electrophoretic mobility shift assay improved for the quantitative analysis of protein-DNA complexes. Fluorescent-labeled oligonucleotide probes incubated with nuclear proteins were followed by electrophoresis. The signals for protein-DNA complexes were measured and normalized with fluorescent-labeled marker using fragment analysis software. This assay proved reliable measurement and multiple detection of DNA binding proteins.