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Acephate induced oxidative damage in erythrocytes.
Aarti Thapar1, Rajat Sandhir, Ravi Kiran
1Department of Biochemistry, Panjab University, Chandigarh 160014, India.
Indian Journal of Experimental Biology
|February 25, 2003
Summary
Acephate exposure in rats decreased key enzyme activities and glutathione levels, causing oxidative stress and red blood cell shape changes. This highlights acephate
Area of Science:
- Toxicology
- Biochemistry
- Hematology
Background:
- Pesticides like acephate are widely used in agriculture.
- Understanding their toxicological effects on animal systems is crucial.
- Erythrocytes are vital for oxygen transport and susceptible to oxidative damage.
Purpose of the Study:
- To investigate the impact of oral acephate administration on rat erythrocytes.
- To assess biochemical and morphological changes in erythrocytes following acephate exposure.
Main Methods:
- Male rats were orally administered acephate (360 mg/kg) daily for 15 days.
- Erythrocyte enzyme activities (acetyl cholinesterase, G6PD, GST, GR) were measured.
- Glutathione content, lipid peroxidation, and cholesterol/phospholipid ratio were analyzed.
- Red blood cell morphology was examined using scanning electron microscopy.
Main Results:
- Acephate decreased acetyl cholinesterase and glucose-6-phosphate dehydrogenase activities.
- Glutathione-s-transferase and glutathione reductase activities increased.
- Reduced glutathione levels and increased lipid peroxidation indicated oxidative stress.
- Elevated cholesterol/phospholipid ratio and altered erythrocyte morphology were observed.
Conclusions:
- Oral acephate administration induces significant oxidative stress in rat erythrocytes.
- Oxidative stress leads to biochemical alterations and morphological changes in red blood cells.
- Acephate's effects on erythrocytes warrant further investigation regarding its broader toxicological implications.