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Related Experiment Videos

Hyaluronan synthase in trabecular meshwork cells.

T Usui1, F Nakajima, R Ideta

  • 1Department of Ophthalmology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, Japan.

The British Journal of Ophthalmology
|February 25, 2003
PubMed
Summary

Hyaluronan synthase (HAS) expression in trabecular meshwork cells is regulated by growth factors like TGF-beta and PDGF-BB. This regulation impacts hyaluronan production, potentially influencing the aqueous humor outflow environment.

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Area of Science:

  • Ocular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Hyaluronan is present in the trabecular meshwork, playing a role in aqueous humor outflow.
  • Understanding hyaluronan synthase (HAS) regulation is crucial for the pathophysiology of the aqueous outflow environment.

Purpose of the Study:

  • To investigate the expression and regulation of hyaluronan synthase (HAS) in trabecular meshwork cells.
  • To determine the effects of transforming growth factor beta (TGF-beta) and platelet-derived growth factor BB (PDGF-BB) on HAS expression and hyaluronan production.

Main Methods:

  • Utilized cultured bovine trabecular meshwork cells (BTMCs).
  • Examined HAS isoform expression at mRNA and protein levels using RT-PCR, quantitative RT-PCR, and immunohistochemistry.
  • Assessed hyaluronan production via high-performance liquid chromatography (HPLC).

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Main Results:

  • All three HAS isoforms were expressed at the mRNA level in BTMCs.
  • TGF-beta and PDGF-BB significantly upregulated HAS2 mRNA and protein expression.
  • Both TGF-beta and PDGF-BB stimulated hyaluronan production from BTMCs.

Conclusions:

  • Trabecular meshwork HAS expression is vital for maintaining hyaluronan levels in the aqueous outflow pathway.
  • TGF-beta and PDGF-BB regulate HAS expression and subsequent hyaluronan production.
  • Modulating HAS expression in the trabecular meshwork offers a potential strategy for managing the aqueous outflow environment.