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Updated: Aug 8, 2026

Assessment of Mitochondrial Functions and Cell Viability in Renal Cells Overexpressing Protein Kinase C Isozymes
Published on: January 7, 2013
[The effects of cholecystokinin octapeptide on PKC activity in rat cerebral cortex neurocytes]
Peng Xiang1, Mingjun Qiu, Zeli Du
1Lab. of Hormone-receptor, Department of Biochemistry, West China School of Predinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Objective:
To investigate the effects of CCK8 on protein kinase C activity in rat cerebral cortex.
Methods:
The cerebral cortex neurocytes were isolated and used as a model. The effects of CCK8, L-364, 718 and L-365, 260 on PKC activities were detected by using a non-radioactive method.
Results:
CCK8 caused a detectable increase in PKC activity at 10(-11) mol/L, and a peak increase of PKC activity was observed at 10(-5) mol/L (about 4.5 U/mg protein). PKC activity was increased in dose-dependent manner by CCK8(10(-11)-10(-6) mol/L). The CCKB-selective receptor antagonist L-365, 260 with a higher efficiency, and the CCKA-selective receptor antagonist L-364, 718 with a lower efficiency were able to block a maximal effect of CCK8-induced PKC activation.
Conclusions:
CCK8 may regulate PKC activities in rat cerebral cortex through CCKB receptor.
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