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[Study on DNA oxidative damage of O3 aging model in mice]
1Department of Physics, School of Basic Medical Sciences, WCUMS, Chengdu 610041, China.
Objective:
This study was aimed to detect the animals' DNA oxidative damage.
Methods:
A free radical mouse aging model was established by putting the mouse in a device for inhaling quantities of ozone (O3). Single cell gel electrophoresis (SCGE) was used for assessing the splenolymphocytes' DNA damage.
Results:
The appearance rate of comet and the length grade of DNA migration for the young control group (P < 0.05). No significant difference in the degree of DNA damage was observed between the O3 model group and the natural aging group(P > 0.05).
Conclusion:
The DNA damage of O3 animal model is consistent with that of natural aging animal; this animal model DNA damage of O3 animal model is consistent with that of natural aging animal; this animal model and the method of SCGE are useful for future researches on aging and antiaging medicine.